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Updated: 2017 Aug. 1

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Warning – Occasionally the phosphosites shown below in more divergent species may be slightly mis-aligned with our algorithm and the calculated Conservation Score may be higher than shown.
Phosphosite   Conservation Score
Human Protein: NOS3 All Species: 2.73
Human Site: S53 Identified Species: 6
UniProt: P29474 Number Species: 10
    Phosphosite Substitution
    Charge Score: 0.3
Phosphosite
Sequences
Species Species
Scientific Name
UniProt ID NCBI Ref Seq ID AA# Mr(Da) P-Site -7 -6 -5 -4 -3 -2 -1 0 1 2 3 4 5 6 7
Human Homo sapiens P29474 NP_000594.2 1203 133289 S53 L P P A P E H S P P S S P L T
Chimpanzee Pan troglodytes XP_001148238 1153 131137 N54 H N L S K Q Q N E S P Q P L V
Rhesus Macaque Macaca mulatta XP_001083352 1431 160593 D150 K E Q P L A V D G A S G P G N
Dog Lupus familis XP_534695 1431 160627 Q281 N P Y S E K E Q P P A S G K Q
Cat Felis silvestris
Mouse Mus musculus P70313 1202 132862 D53 S P T R A A P D H S P P L T R
Rat Rattus norvegicus Q62600 1202 133271 D53 S P T R P A P D H S P P L T R
Wallaby Macropus eugenll
Platypus Ornith. anatinus XP_001506887 1152 131150 H79 G K K S P E Y H A K P R L P T
Chicken Gallus gallus Q90703 1136 129631 I69 E K H P Q N G I K A S N Q I S
Frog Xenopus laevis NP_001079155 1419 160392 T234 Q V E S N S Q T Q P S M Q K D
Zebra Danio Brachydanio rerio NP_571735 1431 161840 T267 W R K D N M P T V L N N P Y S
Tiger Blowfish Takifugu rubipres
Fruit Fly Dros. melanogaster Q27571 1349 151696 Q184 L S P S P K N Q Q Q P R K M S
Honey Bee Apis mellifera
Nematode Worm Caenorhab. elegans
Sea Urchin Strong. purpuratus
Poplar Tree Populus trichocarpa
Maize Zea mays
Rice Oryza sativa
Thale Cress Arabidopsis thaliana
Baker's Yeast Sacchar. cerevisiae
Red Bread Mold Neurospora crassa
Conservation
Percent
Protein Identity: 100 49.5 49.2 50.3 N.A. 93.7 93.9 N.A. 48.9 46.8 49.6 48.7 N.A. 43.8 N.A. N.A. N.A.
Protein Similarity: 100 64.4 62 63 N.A. 96.1 96 N.A. 63.9 63.6 63.9 62.6 N.A. 57.3 N.A. N.A. N.A.
P-Site Identity: 100 13.3 13.3 26.6 N.A. 6.6 13.3 N.A. 20 6.6 13.3 6.6 N.A. 20 N.A. N.A. N.A.
P-Site Similarity: 100 33.3 13.3 46.6 N.A. 6.6 13.3 N.A. 33.3 26.6 26.6 33.3 N.A. 53.3 N.A. N.A. N.A.
Percent
Protein Identity: N.A. N.A. N.A. N.A. N.A. N.A.
Protein Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Identity: N.A. N.A. N.A. N.A. N.A. N.A.
P-Site Similarity: N.A. N.A. N.A. N.A. N.A. N.A.
Phosphosite
Consensus
Position -7 -6 -5 -4 -3 -4 -5 0 +1 +2 +3 +4 +5 +6 +7
% Ala: 0 0 0 10 10 28 0 0 10 19 10 0 0 0 0 % A
% Cys: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % C
% Asp: 0 0 0 10 0 0 0 28 0 0 0 0 0 0 10 % D
% Glu: 10 10 10 0 10 19 10 0 10 0 0 0 0 0 0 % E
% Phe: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % F
% Gly: 10 0 0 0 0 0 10 0 10 0 0 10 10 10 0 % G
% His: 10 0 10 0 0 0 10 10 19 0 0 0 0 0 0 % H
% Ile: 0 0 0 0 0 0 0 10 0 0 0 0 0 10 0 % I
% Lys: 10 19 19 0 10 19 0 0 10 10 0 0 10 19 0 % K
% Leu: 19 0 10 0 10 0 0 0 0 10 0 0 28 19 0 % L
% Met: 0 0 0 0 0 10 0 0 0 0 0 10 0 10 0 % M
% Asn: 10 10 0 0 19 10 10 10 0 0 10 19 0 0 10 % N
% Pro: 0 37 19 19 37 0 28 0 19 28 46 19 37 10 0 % P
% Gln: 10 0 10 0 10 10 19 19 19 10 0 10 19 0 10 % Q
% Arg: 0 10 0 19 0 0 0 0 0 0 0 19 0 0 19 % R
% Ser: 19 10 0 46 0 10 0 10 0 28 37 19 0 0 28 % S
% Thr: 0 0 19 0 0 0 0 19 0 0 0 0 0 19 19 % T
% Val: 0 10 0 0 0 0 10 0 10 0 0 0 0 0 10 % V
% Trp: 10 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % W
% Tyr: 0 0 10 0 0 0 10 0 0 0 0 0 0 10 0 % Y
% Spaces: 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 % _